| SES # | TOPICS | READINGS |
|---|---|---|
| 1 | Introduction | |
| 2 | G-protein coupled receptors and rhodopsin | Unger, V. M., P. A. Hargrave, J. M. Baldwin, and G. F. X. Schertler. "Arrangement of Rhodopsin Transmembrane α-helices." Nature 389 (1997): 203-206. Palczewski, K., T. Kumasaka, T. Hori, C. A. Behnke, H. Motoshima, B. A. Fox, I. L. Trong, D. C. Teller, T. Okada, R. E. Stenkamp, M. Yamamoto, and M. Miyano. "Crystal Structure of Rhodopsin: A G Protein-Coupled Receptor." Science 289 (2000): 739-745. |
| 3 | Visual cascade part I - Activation of rhodopsin by light | Zhukovsky, E. A., and D. D. Oprian. "Effect of Carboxylic Acid Side Chains on the Absorption Maximum of Visual Pigments." Science 246 (1989): 928-930. Farrens, D. L., C. Altenbach, K. Yang, W. L. Hubbell, and H. G. Khorana. "Requirement of Rigid-body Motion of Transmembrane Helices for Light Activation of Rhodopsin." Science 274 (1996): 768-770. |
| 4 | Visual cascade part II - Rhodopsin and G protein (transducin) interaction | Weiss, E. R., D. J. Kelleher, and G. L. Johnson. "Mapping Sites of Interaction between Rhodopsin and Transducin Using Rhodopsin Antipeptide Antibodies." J Biol Chem 263 (1988): 6150-6154. Natochin, M., M. Moussaif, and N. O. Artemyev. "Probing Mechanism of Rhodopsin-catalyzed Transducin Activation." J Neurochem 77 (2001): 202-210. |
| 5 | Visit to an Atomic Force Microscopy Facility | Fotiadis, D., S. Scheuring, S. A. Müller, A. Engel, and D. J. Müller. "Imaging and Manipulation of Biological Structures with the AFM." Micron 33 (2002): 385-397. Field trip schedule (PDF) |
| 6 | Rhodopsin dimerization | Fotiadis, D., Y. Liang, S. Filipek, D. A. Saperstein, A. Engel, and K. Palczewski. "Rhodopsin Dimers in Native Disc Membranes." Nature 421 (2003): 127-128. Liang, Y., D. Fotiadis, S. Filipek, D. A. Saperstein, K. Palczewski, and A. Engel. "Organization of the G Protein Coupled Receptors Rhodopsin and Opsin in Native Membranes." J Biol Chem 278 (2003): 21655-21662. |
| 7 | Rhodopsin mutations, retinal degeneration and night blindness | Sung, C. H., B. G. Schneider, N. Agarwal, D. S. Papermaster, and J. Nathans. "Functional Heterogeneity of Mutant Rhodopsins Responsible for Autosomal Dominant Retinitis Pigmentosa." Proc Natl Acad Sci 88 (1991): 8840-8844. Jin, S., M. C. Cornwall, and D. D. Oprian. "Opsin Activation as a Cause of Congenital Night Blindness." Nature Neuroscience 6 (2003): 731-735. |
| 8 | Field trip to the Novartis Institutes for BioMedical Research | |
| 9 | Drug addiction - Dopamine receptors and activation mechanism | Lee, S. P., C. H. So, A. J. Rashid, G. Varghese, R. Cheng, A. J. Lanca, B. F. O'Dowd, and S. R. George. "Dopamine D1 and D2 Receptor Co-activation Generates a Novel Phospholipase C-mediated Calcium Signal." J Biol Chem 279 (2004): 35671-35678. Guo, W., L. Shi, M. Filizola, H. Weinstein, and J. A. Javitch. "Crosstalk in G Protein-coupled Receptors: Changes at the Transmembrane Homodimer Interface Determine Activation." Proc Natl Acad Sci 102 (2005): 17495-17500. |
| 10 | Allergies - Histamine receptors | Morisset, S., A. Rouleau, X. Ligneau, F. Gbahou, J. Tardivel-Lacombe, H. Stark, W. Schunack, C. R. Ganellin, Schwartz, and J-M. Arrang. "High Constitutive Activity of Native H3 Receptors Regulates Histamine Neurons in Brain." Nature 408 (2000): 860-864. Gillard, M., C. Van Der Perren, N. Moguilevsky, R. Massingham, and P. Chatelain. "Binding Characteristics of Cetirizine and Levocetirizine to Human H1 Receptors: Contribution of Lys191 and Thr194." Mol Pharmacol 61 (2002): 391-399. Milligan, G., R. A. Bond, and M. Lee. "Inverse Agonism: Pharmacological Curiosity or Potential Therapeutic Strategy?" Trends in Pharmacological Sciences 16, no. 1 (January 1995): 10-13. |
| 11 | How do mutations in chemokine receptors inhibit CCR5-mediated HIV infection? | Benkirane, M., D-Y Jin, R. F. Chun, R. A. Koup, and K-T Jeang. "Mechanism of Transdominant Inhibition of CCR5-mediated HIV-1 Infection by ccr5d32." J Biol Chem 272 (1997): 30603-30606. Hernanz-Falcon, P., J. M. Rodriguez-Frade, A. Serrano, D. Juan, A. D. Sol, S. F. Soriano, F. Roncal, L. Gomez, A. Valencia, C. Martinez-A, and M. Mellado. "Identification of Amino Acid Residues Crucial for Chemokine Receptor Dimerization." Nature Immunology 5 (2004): 216-223. |
| 12 | Sense of smell: Olfactory receptors | Buck, L., and R. Axel. "A Novel Multigene Family May Encode Odorant Receptors: Molecular Basis for Odor Recognition." Cell 65 (1991): 175-187. Oka, Y., M. Omura, H. Kataoka, and K. Touhara. "Olfactory Receptor Antagonism between Odorants." EMBO J 23 (2004): 120-126. Techniques used in the papers: |
| 13 | Sense of taste: Taste receptors | Nelson, G., M. A. Hoon, J. Chandrashekar, Y. Zhang, N. J. P. Ryba, and C. S. Zuker. "Mammalian Sweet Taste Receptors." Cell 106 (2001): 381-390. Moon, S. J., M. Köttgen, Y. Jiao, H. Xu, and C. Montell. "Taste Receptor Required for the Caffeine Response in Vivo." Current Biology 16 (2006): 1812-1817. ———. "Supplemental Data: Taste Receptor Required for the Caffeine Response in Vivo." Current Biology 16 (2006): 1812-1817. |
| 14 | Oral presentations: General discussion and future perspectives |